2-[2-[4-[(2-chloroethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium hydrogen sulphate

Appearance

Yellow tetrazole compound

Solubility

Soluble in water

Application

Widely used in cell viability assays

Usage

Assess cell viability, proliferation, and cytotoxicity

Utility

Assess mitochondrial activity

Mechanism

Reduced by dehydrogenase enzymes in metabolically active cells

Result

Formation of a purple formazan product

Quantification

Spectrophotometrically quantifiable

Fields of Use

Cancer research, drug development, toxicology studies

General Description

The chemical "2-[2-[4-[(2-chloroethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium hydrogen sulphate" is a quaternary ammonium compound commonly known as MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). It is a yellow tetrazole compound that is soluble in water and is commonly used in cell viability assays. MTT is widely employed in research and industrial settings to assess cell viability, proliferation, and cytotoxicity. It is a popular compound for assessing mitochondrial activity due to its reduction by dehydrogenase enzymes in metabolically active cells, resulting in the formation of a purple formazan product that can be quantified spectrophotometrically. MTT has shown utility in numerous fields, including cancer research, drug development, and toxicology studies.