Xylene Cyanole FF

  • Name: Xylene Cyanole FF
  • CAS: 2650-17-1
  • Purity: 99%
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Details

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  • Molecular Formula: C25H27N2NaO6S2
  • Molecular Weight: 538.621
  • Appearance/Colour: dark green or blue-grey crystals 
  • Melting Point: 295 °C 
  • PSA: 152.72000 
  • LogP: 6.59110 

Xylene Cyanole FF(Cas 2650-17-1) Usage

Preparation

4-Formylbenzene-1,3-disulfonic acid (1 Moore) and N-ethyl-2-methylbenzenamine (2 Moore) condensation, garnish with heavy chromate salt-oxalic acid oxidation its product.

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Standard

Light Fastness

Fading

Stain

ISO

2

AATCC

2

General Description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Properties and Applications

bright blue. Soluble in water to blue. Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater Fading Stain Fading Stain Fading Stain ISO 2 2 3-4 2-3 1 2-3 1 AATCC 2 4-5 4-5 2-3 1 2 2

InChI:InChI=1/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;